Stereoscopic insight into the biological microcosm

Abstract

The present article deals with the presentation of the stereoscopic method in light microscopy. The easily applicable tilt-shift technique – based on two stereoscopic semi-images – is presented as a method for producing spatial pictures. The photography of an object from two slightly different perspectives is done by tilting the slide (or the microscope) at a corresponding angle of 2° to 10°. With the help of exemplary pictures, it shall be demonstrated that the spatial picture can provide additional information especially when documenting/recording complex three-dimensional structures. Among other things, due to this circumstance stereophotography could be established as a standard method in light microscopy.